Elimination by Gamma Irradiation of Salmonella spp. and Strains of Staphylococcus aureus Inoculated in Bison, Ostrich, Alligator,and Caiman Meat

Sterile meat was thawed rapidly (in about 5 min) by submerging the package in a
50°C water bath, inoculated with enough cells ( 10 ml/100g of meat) for a final
population of ca. 109 stationary- phase cells per g, and stomached for 90 s in
sterile no.400 polyethylene stomacher bags using a Stomacher® 400.

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Polymerase chain reaction (pcr) for the detection of salmonella in artificially

Thirty-two samples of chicken meat were used. From each sample, 25 grams were weighted in  Stomacher® 400 sterile bags and inoculated with 1 mL of a dilution (10-7,10-8 or 10-9) of S. Enteritidis or S. Typhimurium and 1 mL of the other 18 bacteria pool diluted 10-2 . In two of the samples, only the pool was inoculated. Finally, 225 mL of PBW 1% were added and homogenized in a Stomacher 400 for 30 seconds.

As the results are obtained in a shorter time period than that of microbiological culture, this procedure will be useful in the methodology for detection of Salmonella in chicken.

food microbiology PCR chicken salmonella stomacher 400

Ultimate pH values and bacteriological condition of meat and stress metabolites in blood of transported reindeer bulls

The Stomacher® 400 was used to blend reindeer meat for two minutes.

The study found that at slaughter, there was no difference in ASAT activity, urea and Cortisol concentrations between the two transported groups. However, the plasma ASAT activity and urea concentrations at slaughter were significantly lower in the non-transported group. In both transport groups, the plasma Cortisol concentrations increased during loading onto and unloading from the lorry. Abomasal lesions were observed in all treatment groups. It was concluded that reindeer showed an acute stress response to manual handling and transport.

food microbiology meat quality stress metabolites reindeer stomacher 400

Influence of variations in methodology on populations of Listeria monocytogenes recovered from lettuce treated with sanitizers

Stomaching, homogenizing, or stomaching followed by homogenizing lettuce treated with sanitizers resulted in recovery of similar numbers of L. monocytogenes, indicating that stomaching and homogenizing are equivalent in extracting cells.

food microbiology sanitizer evaluation lettuce listeria monocytogenes stomacher 400

Biomonitoring by combination of immunomagnetic separation and direct epifluorescence filter technique

The Stomacher® 400 is used to support DEFT (Direct Epifluorescence Filter Technique).

Dried apricots and figs were used in the experiments. 10g of fruits were mixed with 90mL of phosphate buffered solution (PBS) in a ´400´ Closure Bag and homogenised in a Stomacher 400 device for 10 minutes.

food microbiology dynabead direct epifluoresence apricot stomacher 400

Genotoxic activity in human faecal water and the role of bile acids: a study using the alkaline comet assay

Faecal samples were collected and kept on ice before processing. Samples
were homogenized in a stomacher for 2 minutes in a Stomacher® 400.

The results of the present study indicate that the comet assay provides a powerful tool to investigate the genotoxicity of faecal water samples in a human colonic tumour cell line.

clinical chemistry oncology faeces bile acids stomacher 400