Sterile meat was thawed rapidly (in about 5 min) by submerging the package in a
50°C water bath, inoculated with enough cells ( 10 ml/100g of meat) for a final
population of ca. 109 stationary- phase cells per g, and stomached for 90 s in
sterile no.400 polyethylene stomacher bags using a Stomacher® 400.
To enumerate L. monocytogenes, 0.1 M phosphate buffer was added to each
stomacher bag (5-g beef slice in 45 ml of phosphate buffer), and the contents were mixed in a Stomacher 400 for 2 minutes.
Thirty-two samples of chicken meat were used. From each sample, 25 grams were weighted in Stomacher® 400 sterile bags and inoculated with 1 mL of a dilution (10-7,10-8 or 10-9) of S. Enteritidis or S. Typhimurium and 1 mL of the other 18 bacteria pool diluted 10-2 . In two of the samples, only the pool was inoculated. Finally, 225 mL of PBW 1% were added and homogenized in a Stomacher 400 for 30 seconds.
As the results are obtained in a shorter time period than that of microbiological culture, this procedure will be useful in the methodology for detection of Salmonella in chicken.
food microbiology PCR chicken salmonella stomacher 400
A Stomacher® 400 was used to blend chicken as part of the study. It was used as sample preparation as well as for the enumeration of living bacteria.
food microbiology heat resistance salmonella chicken broth stomacher 400
The Stomacher® 400 was used to blend reindeer meat for two minutes.
The study found that at slaughter, there was no difference in ASAT activity, urea and Cortisol concentrations between the two transported groups. However, the plasma ASAT activity and urea concentrations at slaughter were significantly lower in the non-transported group. In both transport groups, the plasma Cortisol concentrations increased during loading onto and unloading from the lorry. Abomasal lesions were observed in all treatment groups. It was concluded that reindeer showed an acute stress response to manual handling and transport.
food microbiology meat quality stress metabolites reindeer stomacher 400
Stomaching, homogenizing, or stomaching followed by homogenizing lettuce treated with sanitizers resulted in recovery of similar numbers of L. monocytogenes, indicating that stomaching and homogenizing are equivalent in extracting cells.
food microbiology sanitizer evaluation lettuce listeria monocytogenes stomacher 400
The Stomacher® 400 is used to support DEFT (Direct Epifluorescence Filter Technique).
Dried apricots and figs were used in the experiments. 10g of fruits were mixed with 90mL of phosphate buffered solution (PBS) in a ´400´ Closure Bag and homogenised in a Stomacher 400 device for 10 minutes.
food microbiology dynabead direct epifluoresence apricot stomacher 400
The Stomacher® 400 was used to blend blend tissue for two minutes as part of the study. The study found that sponge sampling is convenient, however, is a highly variable means of removing bacteria.
food microbiology beef stomacher 400
The Stomacher® 400 is used as a key piece of equipment in the USDA methodology for meat species identification.
food chemistry meat species identification stomacher 400
Faecal samples were collected and kept on ice before processing. Samples
were homogenized in a stomacher for 2 minutes in a Stomacher® 400.
The results of the present study indicate that the comet assay provides a powerful tool to investigate the genotoxicity of faecal water samples in a human colonic tumour cell line.
clinical chemistry oncology faeces bile acids stomacher 400