Tissue specimens were homogenized in 3 ml of sterile saline using a Seward Stomacher 80, and the sterility of each vial of saline was confirmed at the time of use by bacterial culture.
These results support the need for a minimum culture incubation period of 13 days to be applied to both aerobic and anaerobic culture media for all periprosthetic specimens. Optimal recovery of infecting organisms from PJI specimens will be an important component in generating a universal definition for PJI due to indolent agents of infection, such as P. acnes.
