Development and Validation of a Quantitative Real-Time PCR Assay Using Fluorescence Resonance Energy Transfer Technology for Detection of Aspergillus fumigatus in Experimental Invasive Pulmonary Aspergillosis

Lung tissue in each individual rabbit was sampled and cultured by excision of a representative region of the lung. Each fragment was weighed individually, placed in sterile stomacher® bags, and homogenized with sterile saline for 15 s per tissue sample in a Stomacher® 80.